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The isolation of microsatellite loci in the Mediterranean fruitfly Ceratitis capitata (Diptera: Tephritidae) using a biotin/streptavidin enrichment technique

Casey, D.G. and Burnell, A.M. (2001) The isolation of microsatellite loci in the Mediterranean fruitfly Ceratitis capitata (Diptera: Tephritidae) using a biotin/streptavidin enrichment technique. Molecular Ecology Notes, 1. pp. 120-122.

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Abstract

The Medfly (Ceratitis capitata) is a polyphagous dipteran pest which has spread from North Africa to the countries of the Mediterranean Basin and has also invaded tropical and subtropical regions throughout the world. Colonizing populations typically possess low levels of genetic variability. Microsatellites provide an effective means of investigating the population structure of such genetically depauperate populations, however, microsatellite markers traditionally require a long phase of development in new taxa. We used a biotin/streptavidin capture technique to isolate microsatellites directly from C. capitata genomic DNA and we describe here the identification of seven polymorphic microsatellite markers in C. capitata

Item Type: Article
Keywords: Ceratitis capitata; enrichment protocol; Medfly; microsatellite;
Subjects: Science & Engineering > Biology
Item ID: 175
Depositing User: Prof. Ann Burnell
Date Deposited: 10 Jan 2005
Journal or Publication Title: Molecular Ecology Notes
Publisher: Blackwell Science Ltd.
Refereed: Yes
URI:

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