NUI Maynooth

NUIM ePrints and eTheses Archive

NUIM Library

Characterisation of the entomopathogenic nematode Heterorhabditk (Nematoda : Heterorhabditidae) from Ireland and Britain by molecular and cross-breeding techniques,and the occurrence of the genus in these islands

Griffin, Christine T. and Joyce, Susan A. and Dix, Ilona and Burnell, Ann M. and Downes, Martin J. (1994) Characterisation of the entomopathogenic nematode Heterorhabditk (Nematoda : Heterorhabditidae) from Ireland and Britain by molecular and cross-breeding techniques,and the occurrence of the genus in these islands. Fundalmental Applied Nematology, 17 (3). pp. 245-253.

[img] Download (1MB)

Abstract

Soil surveys were conducted in Ireland and Britain for Heterorhabditis nematodes. Soil samples taken from sandy locations were baited with Galleria rnellonella larvae. Heterorhabditis was detected at 18/169 sites in Ireland, 21.5 1 sites in the North of Scotland, and 9/20 sites in the South of Wales. All of the positive sites were coastal; the genus was not detected at any of the 40 inland sites sampled. All of the 76 isolates recovered in these surveys were identified, using isoelectric focusing, DNA estriction and cross-breeding methods, as belonging to the Irish Group of Heterorhabditis. No member of the North-West European Group of Heterorhabditis was recovered. However, an isolate recovered by other workers and originating in the south of England was identified as belonging to that group. Members of the Irish Group did not generally interbreed with embers of the "E Group from either England or continental Europe, though fertile infective juveniles resulted in a minority (3/15) of intergroup crosses.

Item Type: Article
Keywords: Heterorhabditis; entomopathogenic nematode; distribution; characterisation; cross-breeding; isoelectric focusing; DNA restriction;
Subjects: Science & Engineering > Biology
Item ID: 185
Depositing User: Prof. Ann Burnell
Date Deposited: 26 Jan 2005
Journal or Publication Title: Fundalmental Applied Nematology
Publisher: Gauthier-Villars
Refereed: Yes
URI:

    Repository Staff Only(login required)

    View Item Item control page

    Document Downloads

    More statistics for this item...