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Electrophoretic field gradient focusing with on-column detection by fluorescence quenching

Ansell, Richard J. and Tunon, Pilar Gonzalez and Wang, Yating and Myers, Peter and Ivory, Cornelius F. and Keen, Jeffrey N. and Findlay, John B. C. (2009) Electrophoretic field gradient focusing with on-column detection by fluorescence quenching. Analyst, 134 . pp. 226-229. ISSN 0003-2654

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Abstract

Native, uncoloured, proteins can be focused in a column containing a fluorescent packing material, using hydrodynamic flow and a counteracting non-linear electric field, and imaged along the length of the channel by fluorescence quenching.

Keywords:Electrophoretic; field gradient focusing; on-column detection; fluorescence quenching;
Subjects:Science & Engineering > Biology
ID Code:2070
Deposited By:Professor John Findlay
Deposited On:10 Aug 2010 16:06
Journal or Publication Title:Analyst
Publisher:Royal Society of Chemistry
Refereed:Yes
URL:http://pubs.rsc.org/en/Journals/JournalIssues/AN

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