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    Identification, cloning, and functional expression of three glutathione transferase genes from Aspergillus fumigatus


    Burns, Claire and Geraghty, Rachel and Neville, Claire and Murphy, Alan and Kavanagh, Kevin and Doyle, Sean (2005) Identification, cloning, and functional expression of three glutathione transferase genes from Aspergillus fumigatus. Fungal Genetics and Biology, 42 (4). pp. 319-327. ISSN 1087-1845

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    Abstract

    Analysis of the genome of the human pathogen, Aspergillus fumigatus, revealed the presence of several putative glutathione transferase (GST) open reading frames. Three A. fumigatus GST genes, termed gstA, B, and C, were cloned and recombinant proteins expressed in Escherichia coli. Functional analysis of recombinant gstA–C conWrms that the enzymes exhibit GST activity and glutathione peroxidase activity. RT-PCR conWrmed low basal expression of gstA and gstC which was markedly up-regulated (at least 4£– 10£) in the presence of either H2O2 or 1-chloro-2,4-dinitrobenzene (CDNB). GstB expression was only observed in the presence of CDNB. These results demonstrate for the Wrst time the existence of three functional GSTs in A. fumigatus and strongly suggest a role for these enzymes in the response of the organism to both oxidative stress and xenobiotic presence.

    Item Type: Article
    Keywords: GST; MALDI-TOF; Xenobiotic resistance; Oxidative stress; Fungal;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 230
    Identification Number: https://doi.org/10.1016/j.fgb.2005.01.001
    Depositing User: Dr. Sean Doyle
    Date Deposited: 14 Jul 2005
    Journal or Publication Title: Fungal Genetics and Biology
    Publisher: Elsevier Inc.
    Refereed: Yes
    Funders: Irish Research Council for Science Engineering and Technology (IRCSET)
    URI:
    Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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