Identification, cloning, and functional expression of three glutathione transferase genes from Aspergillus fumigatus
Burns, Claire and Geraghty, Rachel and Neville, Claire and Murphy, Alan and Kavanagh, Kevin and Doyle, Sean (2005) Identification, cloning, and functional expression of three glutathione transferase genes from Aspergillus fumigatus. Fungal Genetics and Biology, 42 (4). pp. 319-327. AbstractAnalysis of the genome of the human pathogen, Aspergillus fumigatus, revealed the presence of several putative glutathione transferase
(GST) open reading frames. Three A. fumigatus GST genes, termed gstA, B, and C, were cloned and recombinant proteins
expressed in Escherichia coli. Functional analysis of recombinant gstA–C conWrms that the enzymes exhibit GST activity and glutathione
peroxidase activity. RT-PCR conWrmed low basal expression of gstA and gstC which was markedly up-regulated (at least 4£–
10£) in the presence of either H2O2 or 1-chloro-2,4-dinitrobenzene (CDNB). GstB expression was only observed in the presence of
CDNB. These results demonstrate for the Wrst time the existence of three functional GSTs in A. fumigatus and strongly suggest a role
for these enzymes in the response of the organism to both oxidative stress and xenobiotic presence. | Keywords: | GST; MALDI-TOF; Xenobiotic resistance; Oxidative stress; Fungal |
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| Subjects: | Science & Engineering > Biology |
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| ID Code: | 230 |
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| Deposited By: | Dr. Sean Doyle |
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| Deposited On: | 14 Jul 2005 |
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| Journal or Publication Title: | Fungal Genetics and Biology |
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| Publisher: | Elsevier Inc. |
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| Refereed: | Yes |
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