The expression of selected non-ribosomal peptide synthetases in Aspergillus fumigatus is controlled by the availability of free iron
Reiber, Kathrin and Reeves, Emer P. and Neville, Claire M. and Winkler, Robert and Gebhardt, Peter and Kavanagh, Kevin and Doyle, Sean (2005) The expression of selected non-ribosomal peptide synthetases in Aspergillus fumigatus is controlled by the availability of free iron. FEMS Microbiology Letters, 248 (1). pp. 83-91.
Three non-ribosomal peptide synthetase genes, termed sidD, sidC and sidE, have been identified in Aspergillus fumigatus. Gene expression analysis by RT-PCR confirms that expression of both sidD and C was reduced by up to 90% under iron-replete conditions indicative of a likely role in siderophore biosynthesis. SidE expression was less sensitive to iron levels. In addition, two proteins purified from mycelia grown under iron-limiting conditions corresponded to SidD (200 kDa) and SidC (496 kDa) as determined by MALDI ToF peptide mass fingerprinting and MALDI LIFT-ToF/ToF. Siderophore synthetases are unique in bacteria and fungi and represent an attractive target for antimicrobial chemotherapy.
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