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Proteomic analysis of the mitochondria-enriched fraction from diabetic rat skeletal muscle

Mullen, Edel and Ohlendieck, Kay (2011) Proteomic analysis of the mitochondria-enriched fraction from diabetic rat skeletal muscle. Journal of Integrated Omics, 1 (1). pp. 108-114. ISSN 2182-0287

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Abstract

Mitochondrial dysfunction in muscle has been implicated to play a causative role or being an indirect consequence of insulin resistance in type-2 diabetes. In order to investigate potential diabetes-related alterations in the mitochondrial proteome of muscle, we have carried out a mass spectrometry-based proteomic analysis of the gastrocnemius muscle from normal versus diabetic Goto-Kakizaki rats. A generally perturbed protein expression pattern was observed in the mitochondria-enriched fraction from diabetic muscle. Various mitochondrial markers, including NADH dehydrogenase, cytochrome b-c1 complex and isocitrate dehydrogenase were reduced in diabetic preparations. Isoforms of pyruvate dehydrogenase and ATP synthase exhibited differential changes in their abundance. The altered protein expression levels of these key metabolic enzymes might trigger a diabetes-dependent decrease in mitochondrial oxidative phosphorylation levels. The proteomic findings presented here support the idea that mitochondrial abnormalities are involved in the molecular pathogenesis of type-2 diabetes and may be crucial for the development of insulin resistance.

Item Type: Article
Additional Information: Research was supported by a project grant from the Irish Health Research Board (HRB-RP/2005/3) and an equipment grant from the Higher Education Authority (HEA-RERGS- 07-NUIM).
Keywords: Diabetes; DIGE; Goto-Kakizaki Rat; Mitochondria; Muscle Proteomics;
Subjects: Science & Engineering > Biology
Item ID: 2465
Depositing User: Prof. Kay Ohlendieck
Date Deposited: 08 Mar 2011 11:37
Journal or Publication Title: Journal of Integrated Omics
Refereed: Yes
URI:

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