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Pre-exposure to yeast protects larvae of Galleria mellonella from a subsequent lethal infection by Candida albicans and is mediated by the increased expression of antimicrobial peptides

Bergin, David and Murphy, Lisa and Keenan, Joanne and Clynes, Martin and Kavanagh, Kevin (2006) Pre-exposure to yeast protects larvae of Galleria mellonella from a subsequent lethal infection by Candida albicans and is mediated by the increased expression of antimicrobial peptides. Microbes and Infection. (In Press)

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Abstract

Pre-exposure of the larvae of Galleria mellonella to Candida albicans or Saccharomyces cerevisiae protects against a subsequent infection with 106 C. albicans cells. This protection can also be induced by exposing larvae to glucan or laminarin prior to the administration of the potentially lethal inoculum. Analysis of the expression of genes coding for galiomicin, a defensin in G. mellonella, a cysteine-rich antifungal peptide gallerimycin, an iron-binding protein transferrin and an inducible metalloproteinase inhibitor (IMPI) from G. mellonella demonstrated increased expression, which is at its highest after 24 h of the initial inoculum. Examination of the expression of proteins in the insect haemolymph using 2D electrophoresis and MALDI TOF analysis revealed an increased expression of a number of proteins associated with the insect immune response to infection 24 h after the initial exposure. This study demonstrates that the larvae of G. mellonella can withstand a lethal inoculum of C. albicans if pre-exposed to a non-lethal dose of yeast or polysaccharide 24 h previously which is mediated by increased expression of a number of antimicrobial peptides and the appearance of a number of peptides in the challenged larvae.

Item Type: Article
Keywords: Galleria mellonella; Antimicrobial proteins; Host defence; 2D gel electrophoresis; MALDI TOF analysis; Gene expression
Subjects: Science & Engineering > Biology
Item ID: 355
Depositing User: Dr. Kevin Kavanagh
Date Deposited: 11 Aug 2006
Journal or Publication Title: Microbes and Infection
Publisher: Elsevier
Refereed: Yes
URI:

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