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Skeletal muscle tissue from the Goto-Kakizaki rat model of type-2 diabetes exhibits increased levels of the small heat shock protein Hsp27

Mullen, Edel and O'Reilly, Elaine and Ohlendieck, Kay (2011) Skeletal muscle tissue from the Goto-Kakizaki rat model of type-2 diabetes exhibits increased levels of the small heat shock protein Hsp27. Molecular Medicine Reports, 4. pp. 229-236. ISSN 1791-2997

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Abstract

In order to increase our understanding of diabetes-related muscle weakness, we carried out a mass spectrometry-based proteomic analysis of skeletal muscle preparations from the Goto-Kakizaki rat model of type-2 diabetes. Fluorescence difference in-gel electrophoresis was performed to determine potential differences in the global protein expression profile of muscle extracts. Besides changes in contractile proteins and metabolic enzymes, the abundance of the small stress proteins αB-crystallin and Hsp27 was significantly increased. The up-regulation of the low-molecular-mass heat shock protein Hsp27 was confirmed by an alternative fluorescent staining method of two-dimensional gels and immunoblotting. The observed protein alterations in the cellular stress response, distinct metabolic pathways, regulatory mechanisms and the contractile apparatus might be directly or indirectly associated with peripheral resistance to insulin signalling, making these newly identified muscle proteins potential biomarkers of type-2 diabetes. Increased levels of molecular chaperones suggest considerably enhanced cellular stress levels in diabetic muscle fibres.

Item Type: Article
Additional Information: The definitive version of this article is available at http://www.spandidos-publications.com/mmr/ DOI: 10.3892/mmr.2011.437 . Research was supported by a Hume Scholarship from NUI Maynooth, a project grant from the Irish Health Research Board (HRB-RP/2005/3) and an equipment grant from the Higher Education Authority (HEA-RERGS-07-NUIM).
Keywords: difference in-gel electrophoresis; Goto-Kakizaki rat; Hsp27; muscle proteomics; type-2 diabetes;
Subjects: Science & Engineering > Biology
Item ID: 3597
Depositing User: Prof. Kay Ohlendieck
Date Deposited: 24 Apr 2012 15:28
Journal or Publication Title: Molecular Medicine Reports
Publisher: Spandidos Publications
Refereed: Yes
URI:

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