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The organophosphate-degrading enzyme from Agrobacterium radiobacter displays mechanistic flexibility for catalysis

Ely, Fernanda and Hadler, Kieran S. and Gahan, Lawrence R. and Guddat, Luke W. and Ollis, David L. and Schenk, Gerhard (2010) The organophosphate-degrading enzyme from Agrobacterium radiobacter displays mechanistic flexibility for catalysis. Biochemical Journal, 432 (3). pp. 565-573. ISSN 0264-6021

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Abstract

The OP (organophosphate)-degrading enzyme from Agrobacterium radiobacter (OpdA) is a binuclear metallohydrolase able to degrade highly toxic OP pesticides and nerve agents into less or non-toxic compounds. In the present study, the effect of metal ion substitutions and site-directed mutations on the catalytic properties of OpdA are investigated. The study shows the importance of both the metal ion composition and a hydrogenbond network that connects the metal ion centre with the substrate-binding pocket using residues Arg254 and Tyr257 in the mechanism and substrate specificity of this enzyme. For theCo(II) derivative of OpdA two protonation equilibria (pKa1 ∼5; pKa2 ∼10) have been identified as relevant for catalysis, and a terminal hydroxide acts as the likely hydrolysis-initiating nucleophile. In contrast, the Zn(II) and Cd(II) derivatives only have one relevant protonation equilibrium (pKa ∼4–5), and theμOHis the proposed nucleophile. The observed mechanistic flexibility may reconcile contrasting reaction models that have been published previously and may be beneficial for the rapid adaptation of OP-degrading enzymes to changing environmental pressures.

Item Type: Article
Additional Information: The final version of record is available at http://www.biochemj.org DOI: doi:10.1042/BJ20101054 .
Keywords: Agrobacterium radiobacter; crystal structure; hydrogen bonding; OpdA; organophosphate-degrading enzyme; organophosphate pesticide; site-directed mutagenesis;
Subjects: Science & Engineering > Chemistry
Item ID: 3677
Depositing User: Gary Schenk
Date Deposited: 23 May 2012 15:31
Journal or Publication Title: Biochemical Journal
Publisher: Portland Press
Refereed: Yes
URI:

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