Bernhardt, Paul V. and Schenk, Gerhard and Wilson, Gregory J.
Direct Electrochemistry of Porcine Purple Acid Phosphatase (Uteroferrin).
Cyclic voltammetry of the non-heme diiron enzyme porcine purple acid phosphatase (uteroferrin,
Uf) has been reported for the first time. Totally reversible one-electron oxidation responses (FeIII-FeII f
FeIII-FeIII) are seen both in the absence and in the presence of weak competitive inhibitors phosphate and
arsenate, and dissociation constants of these oxoanion complexes formed with uteroferrin in its oxidized
state (Ufo) have been determined. The effect of pH on the redox potentials has been investigated in the
range 3 < pH < 6.5, enabling acid dissociation constants for Ufo and its phosphate and arsenate complexes
to be calculated.
||Electrochemistry; Porcine Purple Acid Phosphatase; Uteroferrin;
||Science & Engineering > Chemistry
||29 May 2012 09:28
|Journal or Publication Title:
||American Chemical Society
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