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Malonate-bound structure of the glycerophosphodiesterase from Enterobacter aerogenes (GpdQ) and characterization of the native Fe2+ metal-ion preference

Jackson, Colin J. and Hadler, Kieran S. and Carr, Paul D. and Oakley, Aaron J. and Yip, Sylvia and Schenk, Gerhard and Ollis, David L. (2008) Malonate-bound structure of the glycerophosphodiesterase from Enterobacter aerogenes (GpdQ) and characterization of the native Fe2+ metal-ion preference. Acta Crystallographica Section F, 64 (8). pp. 681-685. ISSN 1744-3091

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Abstract

The structure of a malonate-bound form of the glycerophosphodiesterase from Enterobacter aerogenes, GpdQ, has been refined at a resolution of 2.2 A ° to a final R factor of 17.1%. The structure was originally solved to 2.9 A ° resolution using SAD phases from Zn2+ metal ions introduced into the active site of the apoenzyme [Jackson et al. (2007), J. Mol. Biol. 367, 1047–1062]. However, the 2.9 A ° resolution was insufficient to discern significant details of the architecture of the binuclear metal centre that constitutes the active site. Furthermore, kinetic analysis revealed that the enzyme lost a significant amount of activity in the presence of Zn2+, suggesting that it is unlikely to be a catalytically relevant metal ion. In this communication, a higher resolution structure of GpdQ is presented in which malonate is visibly coordinated in the active site and analysis of the native metal-ion preference is presented using atomic absorption spectroscopy and anomalous scattering. Catalytic implications of the structure and its Fe2+ metal-ion preference are discussed.

Item Type: Article
Additional Information: The definitive version of this article is published in Acta Crystallographica Section F, 2008, v.64 n.8, DOI: 10.1107/S1744309108017600
Keywords: Malonate-bound; glycerophosphodiesterase; Enterobacter aerogenes (GpdQ); Fe2+ metal-ion preference;
Subjects: Science & Engineering > Chemistry
Item ID: 3714
Depositing User: Gary Schenk
Date Deposited: 30 May 2012 15:49
Journal or Publication Title: Acta Crystallographica Section F
Publisher: International Union of Crystallography
Refereed: Yes
URI:

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