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Integrated Metabonomic-Proteomic Analysis of an Insect-Bacterial Symbiotic System

Wang, Yulang and Carolan, James C. and Hao, FuHua and Nicholson, Jeremy K. and Wilkinson, Thomas L. and Douglas, Angela E. (2010) Integrated Metabonomic-Proteomic Analysis of an Insect-Bacterial Symbiotic System. Journal of Proteome Research, 9 (3). pp. 1257-1267. ISSN 1535-3893

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Abstract

The health of animals, including humans, is dependent on their resident microbiota, but the complexity of the microbial communities makes these associations difficult to study in most animals. Exceptionally, the microbiology of the pea aphid Acyrthosiphon pisum is dominated by a single bacterium Buchnera aphidicola (B. aphidicola). A 1H NMR-based metabonomic strategy was applied to investigate metabolic profiles of aphids fed on a low essential amino acid diet and treated by antibiotic to eliminate B. aphidicola. In addition, differential gel electrophoresis (DIGE) with mass spectrometry was utilized to determine the alterations of proteins induced by these treatments. We found that these perturbations resulted in significant changes to the abundance of 15 metabolites and 238 proteins. Ten (67%) of the metabolites with altered abundance were amino acids, with nonessential amino acids increased and essential amino acids decreased by both perturbations. Over-represented proteins in the perturbed treatments included catabolic enzymes with roles in amino acid degradation and glycolysis, various cuticular proteins, and a C-type lectin and regucalcin with candidate defensive roles. This analysis demonstrates the central role of essential amino acid production in the relationship and identifies candidate proteins and processes underpinning the function and persistence of the association.

Item Type: Article
Additional Information: The definitive version of this article is available at Wang, Y., Carolan, J.C., Hao, F-H., Nicholson, J., Wilkinson, T.L. and Douglas, A.E. (2010) Integrated metabonomic-proteomic analysis of an insect-bacterial symbiotic system. Journal of Proteome Research, 9 (3):1257-1267. DOI: 10.1021/pr9007392
Keywords: Acyrthosiphon pisum; amino acid metabolism; aphid; differential protein expression; metabonome; proteome;
Subjects: Science & Engineering > Biology
Item ID: 3783
Depositing User: James Carolan
Date Deposited: 03 Jul 2012 16:00
Journal or Publication Title: Journal of Proteome Research
Publisher: American Chemical Society
Refereed: Yes
URI:

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