NUI Maynooth

NUIM ePrints and eTheses Archive

NUIM Library

A Live, Attenuated Bordetella pertussis Vaccine Provides Long-Term Protection against Virulent Challenge in a Murine Model

Skerry, Ciaran M. and Mahon, Bernard P. (2010) A Live, Attenuated Bordetella pertussis Vaccine Provides Long-Term Protection against Virulent Challenge in a Murine Model. Clinical and Vaccine Immunology, 18 (2). pp. 187-193. ISSN 1556-6811

[img] Download (717kB)

Abstract

Despite successful mass vaccination programs, whooping cough remains a significant cause of neonatal mortality. Immunity induced by current vaccines wanes in adolescence, requiring additional immunizations to prevent resurgence. There is a need for a new generation of vaccines capable of conferring long-lasting immunity from birth. Recently, a live, attenuated whooping cough vaccine, BPZE1, has been developed. Here, an established murine immunization model was used to examine the induction and longevity of immunological memory. In this predictive model, BPZE1 conferred a level of protection against virulent bacterial challenge comparable to that conferred by recovery from prior infection, up to 1 year after immunization. One year after immunization with BPZE1, a pertussis-specific persistent response, with high levels of gamma interferon (IFN-γ), could be detected from spleen cells restimulated with inactivated Bordetella pertussis. BPZE1 induced low levels of interleukin-17 (IL-17) and no IL-10 or IL-5. BPZE1 immunization induced long-lasting, efficacious memory B-cell and specific antibody responses dominated by IgG2a, which were boosted by subsequent challenge. Finally, the antibody induced by BPZE1 was functionally relevant and could clear a virulent B. pertussis infection in antibody-deficient mice following passive transfer. This study suggests that BPZE1 is capable of conferring a high level of long-lived effective protection against virulent B. pertussis.

Item Type: Article
Additional Information: The definitive version of this article is available at Clinical and Vaccine Immunology (2010) Vol.18 No.2 pp.187-193. This work was supported by a grant from the European Commission under the seventh framework, grant agreement number 201502 (CHILD-Innovac).
Keywords: Bordetella pertussis; Live Attenuated Bordetella pertussis Vaccine; Long-Term Protection; Virulent Challenge; Murine Model;
Subjects: Science & Engineering > Biology
Item ID: 3787
Depositing User: Dr. Bernard Mahon
Date Deposited: 04 Jul 2012 15:26
Journal or Publication Title: Clinical and Vaccine Immunology
Publisher: American Society for Microbiology
Refereed: Yes
URI:

Repository Staff Only(login required)

View Item Item control page

Document Downloads

More statistics for this item...