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Phase behavior of mixtures of human lens proteins Gamma D and Beta B1

Wang, Ying and Lomakin, Aleksey and Ogun, Olutayo and Benedek, George B. (2010) Phase behavior of mixtures of human lens proteins Gamma D and Beta B1. Proceedings of the National Academy of Sciences , 107 (30). pp. 13282-13287. ISSN 1091-6490

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Abstract

We have experimentally determined the coexistence surface characterizing the phase behavior of γD-βB1-water ternary solutions. The coexistence surface fully describes the solution conditions, i.e., temperature, protein concentration, and protein composition, at which liquid-liquid phase separation occurs in a ternary solution. We have observed a significant demixing of γD and βB1 i.e., large difference of composition in the two coexisting phases. This demixing suggests that the energy of the γD-βB1 attractive interaction is significantly smaller than the energy of the γD-γD attractive interaction. We also observed the lowering of the phase separation temperature upon increasing of the fraction of βB1 in solution. We provide a theoretical analysis of our experimental data, which enables a quantitative description of our principal experimental findings. In this way, we have evaluated the magnitude and temperature dependence of the relevant interprotein interaction energies. Our findings provide insight into the factors essential for maintaining lens proteins in a single homogeneous phase, thereby enabling lens transparency.

Item Type: Article
Additional Information: The definitive version of this article is available at PNAS Vol.107 No.3(2010), pp. 13282-13287. www.pnas.org/cgi/doi/10.1073/pnas.1008353107
Keywords: cataract; crystallin; phase separation; mixture;
Subjects: Science & Engineering > Chemistry
Item ID: 3834
Depositing User: Jennifer McManus
Date Deposited: 29 Aug 2012 15:42
Journal or Publication Title: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Refereed: Yes
URI:

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