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Evidence of Positive Darwinian Selection in Omp85, a Highly Conserved Bacterial Outer Membrane Protein Essential for Cell Viability

Fitzpatrick, David A. and McInerney, James O. (2005) Evidence of Positive Darwinian Selection in Omp85, a Highly Conserved Bacterial Outer Membrane Protein Essential for Cell Viability. Journal of Molecular Evolution, 61 (1). pp. 90-98.

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Abstract

Omp85is a highly conserved outer membrane protein found in all gram-negative bacteria. It is essential for bacterial cell viability and plays an integral function in the positioning and folding of other outer membrane proteins into the bacterial outer membrane. We have employed a maximum likelihood and a maximum parsimony approach to detect evidence of positive Darwinian selection in Omp85hom ologues from 10 d-proteobacteria and have identified 14 amino acid sites that show evidence of being under the influence of adaptive evolution. Interestingly all sites bar one are concentrated within surface loops of the protein that most likely interact with host immune response or the surrounding environment. Alternatively amino acids within membrane-spanning regions of the protein are found to be under purifying selection most likely as a result of structural constraints.

Item Type: Article
Keywords: Omp85, Positive selection
Subjects: Science & Engineering > Biology
Item ID: 667
Depositing User: Dr. James McInerney
Date Deposited: 17 Aug 2007
Journal or Publication Title: Journal of Molecular Evolution
Publisher: Springer
Refereed: Yes
URI:

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